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The degree of hydrolysis of the samples was firm determined by the L-serine equal approach. The data ended up attained from 3 independent hydrolyses for every roe and protease mixture. Examination of variance (ANOVA) was carried out. Letters a–c suggest major differences between distinct samples prepared with varying enzyme concentrations concurrently level (p < 0.05).
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0 and 45 °C exhibited that every one 3 protease preparations could get to a maximal DH inside of eight h beneath the situations utilized. Alcalase hydrolysates exhibited the ideal antioxidant Houses in DPPH, ABTS, and FRAP antioxidant actions. Concurrently, underneath the very same situations, the DH and antioxidant properties of The 2 types of roe proteins ended up reduced Together with the inclusion of defatting and freeze-drying treatments, potentially as a result of variations in either protein morphology or lack of antioxidant peptides all through lipid extraction, and this calls for further exploration.
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